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电化学阻抗谱表征农产品损伤电学响应特性及检测方法研究进展

Electrochemical impedance spectroscopy for electrical response characterization and detection methods of damage in agricultural products: a review

  • 摘要: 农产品品质安全是保障食品安全和农业经济可持续发展的重要基础。在采收、运输、贮藏及加工过程中,农产品容易受到机械损伤、冻害、过度成熟及微生物侵染等多种因素影响,从而导致组织结构破坏、营养成分变化以及食用品质下降。传统无损检测方法在农产品品质检测中得到广泛应用,但仍存在检测成本较高、光学信号穿透能力有限以及部分技术仅能针对挥发性气体进行检测等问题。近年来,电化学阻抗谱(electrochemical impedance spectroscopy,EIS)技术因能够通过交流电信号获取生物组织内部电学响应信息,从细胞层面反映农产品组织结构变化,为农产品损伤检测提供了新的技术途径。该文首次系统整合EIS在7类农产品损伤中的检测参数与模型,明确其技术边界与落地瓶颈,重点介绍了电阻抗检测的基本原理以及试验过程中等效电路模型、电极类型、测量频率和阻抗数据处理方法的选择,并总结了EIS技术在机械损伤、温度胁迫、过度成熟及微生物作用等不同损伤类型检测中的应用进展。最后分析了EIS技术在实际应用中面临的电极差异、农产品个体差异、检测设备小型化以及品质数据库建设等关键问题,并对未来发展方向进行了展望。随着电阻抗检测技术、传感器技术及机器学习算法的不断发展,EIS在农产品损伤检测与品质监测中的应用范围有望进一步拓展,并为相关检测技术的完善与应用提供参考。

     

    Abstract: The quality and safety of agricultural products are fundamental to ensuring food safety and promoting the sustainable development of the agricultural economy. During harvesting, transportation, storage, and processing, agricultural products are susceptible to various types of damage, including mechanical damage, freezing injury, over-maturity, and microbial spoilage, which can result in structural degradation, changes in nutritional composition, and deterioration of edible quality. Therefore, the development of rapid, accurate, and non-destructive detection methods for agricultural product damage is of great significance for evaluating quality status and ensuring supply chain safety. Conventional non-destructive detection methods have been widely applied in agricultural product quality assessment. However, they still face several limitations, including relatively high detection costs, limited penetration capability of optical signals, and the fact that some technologies mainly rely on volatile compounds for quality evaluation. Therefore, the development of highly sensitive and deep-level non-destructive detection techniques for monitoring internal tissue changes in agricultural products remains an important research direction. In recent years, electrochemical impedance spectroscopy (EIS) has attracted increasing attention due to its capability of acquiring electrical response information from biological tissues through alternating current excitation and reflecting structural changes in agricultural products at the cellular level, thereby providing a promising approach for damage detection of agricultural products. This review systematically integrates the detection parameters and modeling approaches of EIS for seven categories of agricultural product damage, including mechanical damage, freezing injury, heat injury, microbial spoilage, dehydration shrinkage, over-maturity, and meat spoilage, and clarifies its technical applicability and practical limitations. The basic principles of impedance detection are introduced, with emphasis on the selection of equivalent circuit models, electrode types, measurement frequencies, and impedance data processing methods. Furthermore, the effects of different detection conditions on impedance signal acquisition and model performance are analyzed. The application progress of EIS in different damage detection scenarios is summarized, and the relationships among damage-induced tissue structural changes, electrical response characteristics, and detection model construction strategies are discussed, providing theoretical support for further application of EIS technology in agricultural product quality assessment. Finally, the key challenges restricting the practical application of EIS technology are analyzed, including electrode variability, individual differences among agricultural products, miniaturization of detection devices, and the establishment of quality databases. Future development directions are also discussed. The standardization of electrode structures and measurement parameters, the development of portable detection devices suitable for complex environments, and the integration of multi-source data analysis methods are considered important directions for promoting the transformation of EIS technology from laboratory research to practical applications. With continuous advances in impedance measurement technologies, sensor technologies, and machine learning algorithms, the application scope of EIS in agricultural product damage detection and quality monitoring will be further expanded. This progress will promote the development of detection methods toward rapid, accurate, and intelligent evaluation, while facilitating standardized measurement protocols, reliable data interpretation, and large-scale quality monitoring across different agricultural products and application scenarios, thereby providing theoretical references and technical support for agricultural product quality assessment, postharvest quality control, and the improvement and practical implementation of intelligent detection technologies.

     

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