Abstract: In order to prevent and control verticillium wilt of soil-borne cotton and to promote cotton growth, the technology of increasing bacteria and fermentation is the important research content. Starting from the diversity of rhizosphere microorganisms and endophytes of cotton, the microorganisms promoting growth and preventing disease of cotton were isolated and screened, and the effective resource strains for promoting growth and preventing disease of cotton were found. The resource strains were used to develop growth promotion and disease prevention bacterial fertilizer, and field test and demonstration were carried out to provide ecological barrier and nutrient source for cotton to resist disease and increase yield.