Abstract: In this paper, the value equivalent factor of ecosystem services per unit area, modified by multiple factors, was used to estimate the value of 9 ecosystem services in 5 periods from 2000 to 2020 in southwest China. The results show that the main types of land use are forest and grassland. The total ESV from 2000 to 2020 were showing a trend of first decline and then increase. In terms of ecosystem types, forest has the highest. In terms of ES categories, hydrological regulation is the highest. ESV has been increasing from west to east, and with the implementation of ecological protection policies and people’s awareness, the quality of the ecological environment and the ecological situation has been improved.