Abstract: In order to study the specific effects of different specifications of corncob fermentation materials on the Pleurotus ostreatus cultivation, four different specifications of corncob fermentation materials were used as Pleurotus ostreatus cultivation materials.The results showed that: using 0.1～0.4 cm and 0.4～1.3 cm diameter corncobs, the cultivation success rate was higher than 95%, the speed of successfully planting Pleurotus ostreatus increased rapidly, and the biological efficiency exceeded 110%; the corncob diameters were 1.4～2.6 cm and 2.7～3.2 cm, the fermentation reaction temperature is low, and the successful cultivation rate drops to79.11% and 59.11%, respectively. The growth of Pleurotus ostreatus is relatively slow, and the biological efficiency is only 86.3%and 75.36%. The results showed that the fermented corncob with a diameter of 0.1～1.3 cm was the best material for planting Pleurotus ostreatus.