Abstract: In order to investigate the effects of different fertilizers on the growth of Eucalyptus seedlings, Eucalyptus Guanglin No.29 in Guigang area was selected as the research object to investigate the tree height, ground diameter and biomass growth of Eucalyptus under different fertilization treatments. The results showed that different fertilization treatments promoted the growth of Eucalyptus seedling height, ground diameter and biomass, and treatment 4 had the best effect on Eucalyptus seedling height growth. Treatment 3 and treatment 4 had higher promoting effect on the diameter growth of Eucalyptus seedling forest. According to the proportion of fresh biomass distribution in different parts under different fertilization treatments, the proportion of fresh biomass in trunk and leaf was higher, while the proportion of fresh biomass in bark was lower. Among different fertilization treatments, the highest fresh biomass in trunk, branch, bark and leaf was all under treatment 3, except that the fresh biomass in root was higher under treatment 4.