Abstract: With the increase of urban domestic sewage discharge, the effective treatment of domestic sewage has become an important problem that needs to be solved urgently. Based on this, combined with the research and application of various new treatment processes, the improved biological rotary disk process was used to enrich and screen aerobic denitrifying bacteria, and the community structure and diversity of the highly efficient denitrifying aerobic bacteria were analyzed, which provided a basis for new biological denitrification and theoretical support for the application of highly efficient denitrifying aerobic bacteria in practical research in the future.