Abstract: This study introduces a bee breeding box suitable for extremely small parasitic wasps, which can solve the problems of poor airtightness, no ventilation holes, and difficulty in adding hosts in the prior art. In this design, the card slot structure is tightly connected, and the pupal channel is designed to prevent the bee from running out when the box cover is opened when the pupal is added. The gauze structure is designed to ensure ventilation during the feeding process. This design can effectively prevent parasitic bees from running out during the process of bee propagation, and is simpler than the previous process.