Abstract: Building high-order, innovative and challenging "golden course" is the key to improve the quality of talents training in universities. The online and offline blended curriculum is one of the main modes to construct the "golden course". On the basis of analyzing the characteristics and teaching status of plant chemical protection courses, an online teaching platform based on small-scale restricted online courses was constructed, teaching activities before, during and after class were designed, a multiple evaluation system involving teachers and students was established, online teaching and offline teaching were organically integrated, and a mixed online and offline golden course was constructed and implemented in teaching. It effectively improves the teaching effect of the course and provides a reference for the construction of online and offline mixed "golden course" in agricultural colleges.