Abstract: In order to analyze the prevalence of porcine pleuropneumonia actinomycetes in large-scale farms and free-range households in a certain area and their resistance to commonly used antibiotics in clinical practice, the paper collected 170 throat swabs,nose swabs and lung tissues of dead pigs of different ages suspected to be infected with porcine pleuropneumonia actinomycetes in large-scale farms and free-range households in a certain area from 2021 to 2023. The actinomycetes porcine pleuropneumoniae were identified by isolation and culture, morphological observation, biochemical identification and PCR. The drug resistance of the isolates was detected by K-B drug sensitive disk method. The results showed that 65 strains of actinomyces pleuropneumoniae were isolated from 170 samples. The resistance rates of 65 strains of actinomyces pleuropneumoniae isolated to 10 drugs such as neomycin,sulfamethoxil and gentamicin were above 50.8%, and the resistance rates of 8 drugs such as cefotaxime, ceftiofur and ciprofloxacin were 6.2%～33.8%, providing reference for the prevention and control of salmonellosis of piglets in this area and rational drug use.