Abstract: Three treatments were designed with black mulch, transparent mulch and black mulch, and the effects of different cultivation methods on the growth of Robinia pseudoacacia seedlings were studied. The results showed that mulching film could effectively shorten the seedling stage and improve the seedling rate of Robinia pseudoacacia. Especially, mulching film could shorten the seedling time by 2.67d and increase the seedling rate by 29.30% compared with that without mulching film. Mulching film can promote the growth of Robinia pseudoacacia seedlings, increase seedling height, ground diameter, DBH, proportion of first-order seedlings,first-order root thickness, and transparent mulching film can also effectively improve the root length and single root quality of Robinia pseudoacacia seedlings. It can be considered that in the process of black locust seedling rearing, it is recommended to use plastic film to cover the ground to improve the survival rate of seedlings and promote the growth of seedlings.