Abstract: Nutrition Diagnosis of Horticultural Plants is an important course in horticulture specialty. Under the background of new agricultural science, Yangtze University has constructed the curriculum system and content of "one center, two systems, three questions" and implemented diversified teaching methods. The reform of teaching methods improved the students’ innovative consciousness and thinking, and made the students’ diagnosis ability of field nutritional deficiency reach 96.18%. The course promoted the students’ development and improved the students’ adaptability under the new agricultural background.