Abstract: The research on the distributing machine of red matsutake can reduce the labor cost on the one hand, and on the other hand, the distributing machine can reasonably arrange the bacteria, efficiently stack the bacteria bed, ensure the uniformity of the stacking, and provide a better growth environment for red matsutake. The study of Pinus densiflora seeder can improve the problems of high missed seeding rate, high damage rate and inconsistent seeding depth caused by the factors such as single seeding device structure, low working efficiency and unable to guarantee the operation quality of the current Pinus densiflora seeder, so as to ensure the efficiency of seeding work. In this paper, the performance of red matsutake seeder is tested by taking the missed sowing rate,damage rate and qualified rate of sowing depth as test indexes. The experiment shows that the missed seeding rate of this seeder is1.91%, the damage rate is 3.62%, the qualified rate of sowing depth is 92.10%.