Abstract: The flipped classroom teaching mode subverts the traditional experimental teaching mode and process, students’ autonomous learning, teachers’ guidance and assistance, flexible in the process of knowledge internalization and absorption, improves students’ interest in autonomous inquiry learning and improves students’ autonomous learning literacy. Relying on the national characteristic specialty of plant protection and the provincial experimental teaching demonstration center of Qingdao Agricultural University,this paper applies the flipped classroom model to the teaching process of "agricultural plant pathology experiment", which promotes the learning efficiency and teaching effect, and cultivates high-quality agricultural scientific and technological talents.