Abstract: Horticultural Plant Breeding is one of the required courses for horticulture majors in colleges and universities. It is of great significance to cultivate students’ comprehensive application and practical operation ability. In this paper, the present situation of theoretical and experimental teaching of horticultural plant breeding is summarized, and its shortcomings and shortcomings are discussed. In view of the problems existing in the horticultural plant breeding course, the online course platform is used to achieve a hybrid teaching method of online and offline, and various teaching modes are used to break the problems such as the limitations of time and space in students’ learning, the singleness of teaching content and lag. The purpose is to improve the teaching quality and the learning efficiency of students by mobilizing students’ subjective initiative and enthusiasm.