Abstract: Grain rust disease caused by rust fungi is one of the major diseases threatening world food security for a long time. The identification and analysis of the host host of rust fungus can clarify the life history of rust fungus and further explore the influence of the sexual recombination process on the pathogenicity of pathogens. On the other hand, it can improve the regional epidemiology of rust fungus and provide a theory for the comprehensive prevention and control of rust.