Abstract: Hemeizhan, the main rice variety in Puning area of Guangdong Province, was selected as an experimental object to study the effects of organic fertilizer replacement on rice agronomic traits, yield, leaf SPAD and economic benefits. The experimental results showed that H1and H2were 3.8% and 8.3% higher than H0, respectively. With the increase of organic fertilizer replacement amount, SPAD value and economic benefit of rice leaves gradually increased, and reached the highest peak in 20% organic fertilizer group, and then showed a downward trend. The results showed that organic fertilizer could replace part of chemical fertilizer, improve rice yield and quality, and achieve the purpose of reducing fertilizer amount and increasing efficiency.