Abstract: For the knowledge points of photosynthesis that are difficult for students to understand, combined with the reaction process of photorespiration, game learning is introduced into the classroom teaching of Plant Physiology. The practical results showed that compared with traditional teaching methods, game-based learning has increased students’ interest in learning, increased classroom participation, and improved their teamwork ability. Students can transfer knowledge well, promote the construction of higherlevel knowledge, and carry out ideological and political education in various aspects. It is a relatively novel classroom teaching attempt.