Abstract: In order to study different concentrations of tribenuron the Blackberrylily Rhizome photosynthetic characteristics, using a randomized complete block design, in order the field cultivation Blackberrylily Rhizome as the experimental material, processed with different concentrations of tribenuron Blackberrylily Rhizome leaves at spraying on the third day determination of Blackberrylily Rhizome chlorophyll content（Chl）, net photosynthetic rate（Pn）, transpiration rate（Tr）, stomatal conductance（Gs）, inter cellular CO2 concentration（Ci） and other indicators. The results showed that: herbicide treatment Blackberrylily Rhizome the chlorophyll content, net photosynthetic rate, transpiration rate, stomatal conductance has emerged as adownward trend; inter cellular carbon dioxide concentration showed an increasing trend; resulting tribenuron Blackberrylily Rhizome the safety and photosynthetic characteristics of an impact.