Abstract: In order to screen out effective, low-toxicity and low-residue biological agents for the control of Tuta absoluta, three biological agents, namely 6% ethyl polycidin suspension（Iverucidin）, 31% Avicidin suspension and 20% chlorfenamide suspension were selected to conduct drug efficacy tests in Aksu City in 2022. The results showed that the three biological agents had certain control and inhibition effects on the larvae of Tuta absoluta. From the point of view of control effect, 20% chlorobenzamide suspension had the highest quick-acting, persistent and control effect, and the control effect was 90.32% after 14 days. It was followed by 6%ethyl polycidin suspension（Alucidin）, which had good durability at 14 days after administration, and the control effect was 81.49%.In the actual production, the use of 20% chlorobenzamide suspension and 6% ethyl polycidin suspension can achieve better control effect, and slow down the resistance of Tuta absoluta.