Abstract: Long-term application of chemical fertilizer in tea gardens is easy to cause soil compaction, which leads to the decrease of both tea yield and quality. The effect is good when black fungus culture-medium waste is used as base fertilizer. In order to provide a theoretical basis for the implementation of new fertilization methods in tea gardens, the effects of replacing commercial organic fertilizer with fungus culture-medium waste on yield, fertility and tea quality of tea gardens were compared. The results showed: The application of "edible bacteria-based waste + compound fertilizer + urea" in tea gardens had better effect, and its yield and quality were slightly higher than that of "commercial organic fertilizer + compound fertilizer + urea", and significantly better than that of inorganic fertilizer（conventional fertilizer）. The organic matter content of the soil can be significantly increased and the microenvironment of the soil of tea garden can be effectively improved by proper decomposition of the edible bacteria-based waste to the field.