Abstract: In the forest ecosystem, wild animals and plants as an indispensable part must be strengthened protection. In the past period of time, in order to promote the development of rural economy, vigorously carry out comprehensive agricultural development, the ecological resources of nature and the living environment of wild animals have been damaged, resulting in the gradual deterioration of the ecological environment. This paper discusses the significance of wildlife protection, combined with the status quo of wildlife protection in Guangxi Defu County-level nature reserve, puts forward some countermeasures to strengthen the protection of wildlife in Guangxi Defu County-level nature reserve.