Abstract: In this study, the ANS gene was cloned from five different color rapeseed genotypes and full-length cDNA of different copies of ANS gene in the petals were analyzed by semi-quantitative PCR. The results showed that the sequences of BnaA01g12530D and BnaC01g14310D copies of ANS gene were completely consistent with the reference sequence in different color rapeseed, and no significant expression difference was found in the petals. However, the BnaA03g45610D and BnaC07g37670D copies showed sequence diversity in different color rapeseed, and their expression levels in the petals of red and orange rapeseed materials were significantly different from those of other colors of rapeseed.