Abstract: Pasteurella Infection is a common acute and infectious disease in modern large-scale cattle farms, it has the characteristics of strong infectiveness, fast transmission speed and high fatality rate, which has great impact on cattle health and cattle industry development. How to effectively prevent and control Pasteurella Infection has become a hot issue concerned by industry workers and farmers. Based on the analysis of epidemiology and diagnosis of Pasteurella Infection, feasible comprehensive prevention and control measures were put forward.