Abstract: According to the present drawback of the practice course teaching of Automobile Tractor Tectonics and the limitation of the experimental equipment and the experimental site, the 3D virtual reality technology was used in automobile tractor tectonics in the practice teaching. Virtuality and reality combination of practice teaching reform was discussed, according to the different nature and content of practice, the form of practice teaching combining virtuality and reality was determined. Evaluation mode of experiment examination way was reformed, and teaching effect was scientific evaluated. The multi-point collaborative assessment of multiangle and multi-way was designed to build a scientific three-dimensional comprehensive evaluation system. It has been found that virtuality and reality combination of practice teaching reform based on the tractor disassembling educational software make up for the shortcomings of traditional practice. In the actual teaching, the students’ learning enthusiasm was fully mobilized, and good results have been received.