Abstract: Potato is a tuber crop with low reproduction coefficient, and the rational application of soilless cultivation technology will greatly improve the reproduction coefficient. In this experiment, a comparison experiment was carried out between aerosol cultivation（AC） and perlite soilless substrate cultivation（PSC） to form micro-potato with virus-free tissue culture seedlings. The results showed that there were no significant changes in plant height, stem diameter and chlorophyll a content between AC and PSC at the10th day of cultivation, while chlorophyll b content in AC was significantly higher than that in PSC. On the 45th day of cultivation,plant height and sucrose content of AC were significantly higher than PSC, while stem diameter and carotenoid content were significantly lower than PSC. The potato yield per plant of AC was significantly higher than PSC, while the average potato weight per plant was significantly lower than PSC. Although the amount of potatoes produced by AC is significantly higher than that by PSC, PSC is recommended for the production of micro-potatoes in consideration of the amount of potatoes produced by AC, electricity consumption and technical input.