Abstract: To study the effect of replacing fish meal with non grain protein sources on the growth performance of Micropterus salmoides. Five experimental feeds were prepared using fish meal, soybean meal, cotton meal and other raw materials as important protein sources, and crystal amino acids were supplemented to meet the growth impact of the demand for essential amino acids in Micropterus salmoides. The results showed that there were no significant differences in daily gain, fatness, hepatobody ratio, viscerobody ratio, water content, crude protein and crude fat contents of whole fish among all groups（P＞0.05）. With the increase of the proportion of chicken meal replacing fish meal, the optimal addition of crude fat in whole fish was 10%, and the survival rate of replacing 10% fish meal was 100%. The mass gain rate of all groups was significantly different from that of group 5（P＜0.05）, and the mass gain rate of 10% fish meal was higher than that of control group, group 4 and group 5. It can be seen that the replacement of10% fish meal with chicken meal has no effect on the body composition, composition content and growth rate of Micropterus salmoides. The best effect of chicken meal instead of fish meal on the growth of Micropterus salmoides is 10%.