Abstract: In order to clarify the effects of different seed mixes on the control effect and safety of wheat stem base rot disease, the optimal seed mix suitable for the control of wheat stem base rot disease in Longxi region of Gansu Province was selected to help the wheat safety production and grain stability in Longxi region of Gansu Province. Three treatments were designed and studied in Longxi County, Gansu Province from October 2022 to June 2023. The results showed that the wheat could grow safely under the treatment of 27% phenyleth-roxylthianium suspension seed coating agent and Baoqi, Bacillus licheniformis, Bacillus thuringiensis high-efficiency seed mix agent, and the incidence of wheat stem base rot was reduced by 74.24% and 77.27%, respectively, compared with the spray water control, and the control effect reached 76.86% and 78.51%, respectively. Under the treatment of Bacillus licheniformis and Bacillus thuringiensis, the control effect was the best. Under this treatment, the total panicle number, kernel number per spike, thousand kernel weight and yield of wheat were 46.47×10~4 strains/667 m~2, 26.84 grains/ear, 40.16 g and 510.24 kg/667m~2, respectively.