Abstract: Ethanol extracts of three samples were prepared from Ginkgo biloba L. fresh leaves(sample 1), dry leaves(sample 2) and old leaves(sample 3). HPLC-DAD-MS method was used to analyze the pigment compounds in three G. biloba leaves samples. The separation, purification and structure identification of ginkgo leaf pigment compounds in sample 2 and sample 3 were conducted by liquid-liquid extraction, silica gel column chromatography, HPLC preparative chromatography, etc. From the three samples, 24 kinds of pigment compounds in G. biloba leaves were preliminarily speculated, including 17 kinds of chlorophyll compounds and 7 kinds of carotenoid compounds. Ten monomer pigment compounds in G. biloba leaves were isolated and purified from ethanol extract of sample 2 and sample 3. They were identified as β-carotene (A), pheophytin a (B), pheophytin b (C), lutein (D), phy-purpurin 18a (E), pyropheophytin a (F), 13²-OH-pheophytin a (G), 13²-OH-pheophytin a' (H), 13²-OH-pheophytin b (I), and 13²-OH-pheophytin b' (J), respectively, by mass spectrometry, ultraviolet spectroscopy, infrared spectroscopy and proton nuclear magnetic resonance spectroscopy combined with literature comparison. Among them, E, F, G, H, I and J were isolated from G. biloba leaves for the first time.