Abstract: By analyzing the response of the fungal community structure in the facility continuous cropping soil to bio-charcoal, a theoretical foundation was laid for alleviating the obstacles of facility continuous cropping and the application of bio-charcoal in facility continuous cropping cultivation. The grafted cucumber “Der 3” was chosen for the test material based on the greenhouse pot experiment, and the vegetable soil of continuous cropping 20 a, 15 a, 10 a, 5 a, 1 a and 0 a（control） was taken respectively. After adding a certain amount of biochar（20 t/hm~2）, the effects of biochar on fungal community structure and diversity in continuous cropping soils were studied. The results were as follows: the proportion of Basidiomycota and Zygomycota in the soil increased adding a certain amount of biochar to the continuous cropping soil of different years, the chytridiomycota（Chytridiomycota） and other unknown fungal phyla decreased at a certain extent, and it was most obvious in the treatment of 15 a continuous cropping vegetables（L15 J）. The performance was most obvious in the soil（L1 J）; at the same time, the diversity of fungi in soil of continuous cropping for 20 a（L20 J） was improved. The similarity of the number of fungi in each treatment was relatively small, and the specificity of fungi in each treatment was relatively high. With the increase of planting years, the specificity of fungal flora in the soil was increased, and a large number of new species of fungi appeared at 200～400 OTUs, and the emergence rate of new species showed a downward trend at 1 000 OTUs, the fungal species in the soil of continuous cropping 20 a（L20 J） were the most. Those results showed that applying a certain amount of biochar to the soil for different continuous cropping years increased the proportion of beneficial fungal flora in the soil, and improved the fungal richness and diversity in the soil, which may contribute for soil improvement.