猪肉中莱克多巴胺便携式快速筛查装置研究

郭庆辉; 彭彦昆; 庄齐斌; 陈雅惠

猪肉中莱克多巴胺便携式快速筛查装置研究

郭庆辉^1,2,
彭彦昆^1,2,,
庄齐斌^1,2,
陈雅惠^1,2

1. 中国农业大学工学院;
2. 国家农产品加工技术装备研发分中心

基金项目:

国家自然科学基金国际(地区)合作交流项目(32020103016)

详细信息

作者简介:
郭庆辉(1993—),男,博士生,主要从事农产品化学残留拉曼快速无损检测技术研究,E-mail:guoqinghui0606@163.com

通讯作者:
彭彦昆(1960—),男,教授,博士生导师,主要从事农产品无损检测技术与装备研究,E-mail:ypeng@cau.edu.cn

中图分类号: TS251.51;O657.37
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出版历程
- 收稿日期: 2022-06-17
- 刊出日期: 2022-11-17

Design and Experiment of Portable Rapid Screening Device for Ractopamine Detection in Pork

1. College of Engineering, China Agricultural University;
2. National R&D Center for Agro-processing Equipment

摘要

摘要: 莱克多巴胺俗称“瘦肉精”,可被用来饲养牲畜以提高胴体瘦肉率。食用含有莱克多巴胺的畜禽肉或内脏可引起健康问题甚至危害生命。传统的莱克多巴胺检测周期较长，费时费力，不利于实际大范围推广使用。在一些屠宰场往往采用抽检的方式进行检测，存在严重滞后性。研发了一种猪肉中“瘦肉精”莱克多巴胺便携式快速筛查装置，该装置主要包括光谱采集模块、光源模块、控制模块和电源。并基于NI LabVIEW软件开发工具，采用G语言编写猪肉“瘦肉精”智能快速检测的控制软件。首先，在碱性环境下利用乙酸乙酯对猪肉中莱克多巴胺进行提取，并采用表面增强拉曼散射（SERS）方法进行检测。研究对比了不同浓度NaCl水溶液作为聚集剂对莱克多巴胺SERS光谱的影响，结果表明以1 mol/L NaCl为聚集剂的增强效果最好。其次，比较了液滴蒸发对拉曼信号的影响，结果表明在滴加样品后，4 s后采集的拉曼信号较好。然后，制备不同莱克多巴胺含量（1、2、4、6、8、10μg/g）的猪肉样品进行定量分析，采用自动惠塔克拟合算法（AWF）对光谱数据进行预处理，扣除原始拉曼光谱中包含的荧光背景。建立836 cm^-1处SERS强度与猪肉样品中莱克多巴胺含量之间的一元线性回归模型。结果表明，模型具有较好的线性关系，决定系数R²为0.99,均方根误差为0.178μg/g。最后，重新制作一批莱克多巴胺含量相同的猪肉样本，利用研发的装置对猪肉中莱克多巴胺进行检测，预测值与样品标准理化值具有较好的线性关系，决定系数R²为0.99,均方根误差为0.317μg/g。本装置简单便携，价格便宜，检测时间小于1 h,检出限为1μg/g,可以用于猪肉中莱克多巴胺的快速筛查。
- 莱克多巴胺 /
- 瘦肉精 /
- 便携式快速筛查装置 /
- 表面增强拉曼散射
Abstract: Ractopamine is a synthetic β-receptor agonist that can be used to feed livestock to improve lean meat yield. Ractopamine can cause health problems and even endanger life if eaten from livestock, poultry or viscera. Traditional ractopamine detection cycle is long, time-consuming and laborious, which is not conducive to the actual large-scale promotion of use. In some slaughterhouses, sampling inspection is often used for testing which is a serious lag. A portable rapid screening device for ractopamine in pork was developed. The device mainly included spectra acquisition module, light source module, control module, power supply and computer. Based on NI LabVIEW software development tool, G language was used to write the control software of intelligent and rapid detection of pork “clenbuterol”. Firstly, ractopamine was extracted from pork by ethyl acetate in alkaline environment, and detected by SERS method. The effects of several different concentrations of NaCl aqueous solutions as aggregators on SERS spectra of ractopamine were compared. The results showed that 1 mol/L NaCl as aggregator had the best enhancement effect. Secondly, the effect of droplet evaporation on Raman signal was compared. The results showed that the Raman signal was better after 4 s. Then, pork samples with different ractopamine contents（1 μg/g, 2 μg/g, 4 μg/g, 6 μg/g, 8 μg/g and 10 μg/g） were prepared for quantitative analysis. Automatic Whittaker fitting algorithm（AWF） was used for preprocessing, and the fluorescence background contained in the original Raman spectrum was deducted. A linear regression model was established between SERS intensity at 836 cm^-1 and ractopamine content in pork samples. The results showed that the model has a good linear relationship, the correlation coefficient R~2 was 0.99, and the root mean square error was 0.178 μg/g. Finally, a batch of pork samples with the same ractopamine content were prepared, and the device was used to detect ractopamine in pork. The predicted values had a good linear relationship with the standard physicochemical values of the samples, the correlation coefficient R~2 was 0.99, and the root mean square error was 0.317 μg/g. The device was simple, portable, inexpensive, and the detection time was less than 1 h. The detection limit was 1 μg/g. It can be used for rapid screening of ractopamine in pork.
- ractopamine /
- brown meat essence /
- portable rapid screening device /
- surface-enhanced Raman scattering

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参考文献(22)

[1]	刘晓佳,韩世磊,孔香玲,等.盐酸莱克多巴胺-D6新的合成方法研究与结构表征[J].化学试剂,2022,44(4):608-612.LIU Xiaojia,HAN Shilei,KONG Xiangling,et al.Novel synthetic method and characterization of ractopamine-D6 hydrochloride[J].Chemical Reagent,2022,44(4):608-612.(in Chinese)
[2]	JING Huan,OUYANG Huiying,LI Weifeng,et al.Molten salt synthesis of BCNO nanosheets for the electrochemical detection of clenbuterol[J].Microchemical Journal,2022,178:107359.
[3]	SOARES M H,JUNIOR D T V,DE AMORIM R G,et al.Effects of feeding ractopamine hydrochloride with or without supplemental betaine on live performance,carcass and meat quality traits,and gene expression of finishing pigs[J].Meat Science,2022,191:108851.
[4]	农业农村部.2019年动物及动物产品兽药残留监控计划[S].2019.
[5]	DU Wei,ZHAO Gang,FU Qiang,et al.Combined microextraction by packed sorbent and high-performance liquid chromatography-ultraviolet detection for rapid analysis of ractopamine in porcine muscle and urine samples[J].Food Chemistry,2014,145:789-795.
[6]	农业部1025号公告-18—2008.动物源食品中β-受体激动剂残留检测液相色谱-串联质谱法[S].2008
[7]	农业部1031号公告-3—2008.猪肝和猪尿中β-受体激动剂残留检测气相色谱-质谱法[S].2008.
[8]	SN/T 4818—2017 进出口食用动物中莱克多巴胺、沙丁胺醇、盐酸克伦特罗的测定酶联免疫吸附法[S].2017.
[9]	闫帅,李永玉,彭彦昆,等.拉曼检测系统中微量试样自动混匀控制装置设计与试验[J].农业机械学报,2021,52(1):324-332.YAN Shuai,LI Yongyu,PENG Yankun,et al.Design and test of automatic mixing and control device for micro sample in Raman detection system[J].Transactions of the Chinese Society for Agricultural Machinery,2021,52(1):324-332.(in Chinese)
[10]	PHAM T B N,BUI T T T,TRAN V Q,et al.Surface-enhanced Raman scattering (SERS) performance on salbutamol detection of colloidal multi-shaped silver nanoparticles[J].Applied nanoscience,2019,10(3):703-714.
[11]	彭彦昆,田文健,郭庆辉,等.基于增强拉曼光谱的苹果中啶虫脒农药残留的无损定量分析[J].农业工程学报,2021,37(14):310-316.PENG Yankun,TIAN Wenjian,GUO Qinghui,et al.Research on identification and determination of pesticides in apples using Raman spectroscopy[J].Transactions of the CSAE,2021,37(14):310-316.(in Chinese)
[12]	ZHAI Fuli,HUANG Yiqun,LI Chunying,et al.Rapid determination of ractopamine in swine urine using surface-enhanced Raman spectroscopy[J].Journal of Agricultural and Food Chemistry,2011,59(18):10023-10027.
[13]	GUO Qinghui,PENG Yankun,CHAO Kuanglin.Raman enhancement effect of different silver nanoparticles on salbutamol[J].Heliyon,2022,8(6):e9576.
[14]	田文健.苹果典型农药残留便携式无损检测装置的研发[D].北京:中国农业大学,2021.TIAN Wenjian.Apple typical pesticide residue portable nondestructive testing device development[D].Beijing:China Agricultural University,2021.(in Chinese)
[15]	张雷蕾,滕官宏伟,朱诚.便携式水产品多品质参数拉曼检测装置设计与试验[J].农业机械学报,2020,51(增刊2):478-483.ZHANG Leilei,TENG-GUAN Hongwei,ZHU Cheng.Development of portable device for detection on multi-quality parameters of aquatic products by Raman spectroscopy[J].Transactions of the Chinese Society for Agricultural Machinery,2020,51(Supp.2):478-483.(in Chinese)
[16]	FRENS G,KOLLOID Z.Controlled nucleation for regulation of particle-size in monodisperse gold suspensions[J].Nature Physical Science,1972,241(105):20-22.
[17]	房晓倩,彭彦昆,王文秀,等.基于表面增强拉曼的鸡尾酒中苯甲酸钠和山梨酸钾的同时快速检测[J].光谱学与光谱分析,2018,38(9):2794-2799.FANG Xiaoqian,PENG Yankun,WANG Wenxiu,et al.Rapid and simultaneous detection of sodium benzoate and potassium sorbate in cocktail based on surface-enhanced Raman spectroscopy[J].Spectroscopy and Spectral Analysis,2018,38(9):2794-2799.(in Chinese)
[18]	李延,彭彦昆,翟晨.基于拉曼光谱的PE膜包装食用农产品品质检测误差的校正方法[J].光谱学与光谱分析,2018,38(9):2800-2805.LI Yan,PENG Yankun,ZHAI Chen.A Raman spectrum detection method for quality of cucumber covered PE plastic wrap[J].Spectroscopy and Spectral Analysis,2018,38(9):2800-2805.(in Chinese)
[19]	翟晨,彭彦昆,李永玉,等.基于拉曼光谱的苹果中农药残留种类识别及浓度预测的研究[J].光谱学与光谱分析,2015,35(8):2180-2185.ZHAI Chen,PENG Yankun,LI Yongyu,et al.Research on identification and determination of pesticides in apples using Raman spectroscopy[J].Spectroscopy and spectral analysis,2015,35(8):2180-2185.(in Chinese)
[20]	GUO Zhiming,CHEN Ping,WANG Mingming,et al.Rapid enrichment detection of patulin and alternariol in apple using surface enhanced Raman spectroscopy with coffee-ring effect[J].LWT,2021,152:112333.
[21]	CHEN Yang,WU Lihang,CHEN Yanhua,et al.Determination of mercury (Ⅱ) by surface-enhanced Raman scattering spectroscopy based on thiol-functionalized silver nanoparticles[J].Microchimica Acta,2012,177(3-4):341-348.
[22]	BI Shuyun,SHAO Di,YUAN Yue,et al.Sensitive surface-enhanced Raman spectroscopy (SERS) determination of nitrofurazone by β-cyclodextrin-protected AuNPs/γ-Al2O3 nanoparticles[J].Food Chemistry,2022,370:131059.