Abstract: Aiming to investigate the effect mechanism of hypoxia-inducible factor-1α（HIF-1α） on glycolysis capacity of postmortem bovine muscle, the bovine M. Longissimus dorsi was selected as the experimental material, the cellular aggregation and nuclear migration of HIF-1α, expression of HIF-1α and glycogen transporter-1, glycolysis rate-limiting enzymes activities, glycolytic potential, and pH values were determined at different time points of postmortem aging（0 h, 6 h, 12 h, 24 h, and 48 h） in the control group and 3-（5′-hydroxymethyl-2′-furyl）-1-benzyl indazole（YC-1）-treated group. The results showed that HIF-1α was aggregated and translocated to the cell nucleus in bovine muscle at the early stage of postmortem aging. The expression levels of HIF-1α were increased by 1.20-fold at 0～6 h（P＜0.05）, which were significantly higher than those in the YC-1-treated group at 6～48 h（P＜0.05）. With the steady accumulation of HIF-1α, the expression levels of GLUT-1 were significantly increased by 1.91-fold within 0～6 h in the control group（P＜0.05）. The hexokinase（HK） and phosphofructokinase（PFK） activities were significantly elevated to 197.43 U/g protein and 27.33 U/g protein at 0～6 h and 0～12 h, respectively（P＜0.05）. As for the glycolysis capacity, the glycogen consumption and lactate accumulation rates were significantly higher in the control than those in the YC-1-treated group（P＜0.05）. The glycolytic potential was significantly increased by 1.10-fold at 0～12 h（P＜0.05） and was significantly higher than that of YC-1 treatment from 6 h to 24 h（P＜0.05）. Meanwhile, pH values in the control group were significantly decreased to the ultimate pH（pHu） at 0～12 h（P＜0.05）, which was 12 h earlier than that in the YC-1-treated group. The results indicated that HIF-1α played an important role in enhancing the glycolytic capacity of bovine muscle by inducing GLUT-1 expression and elevating the activities of glycolysis rate-limiting enzymes at the early stage of postmortem aging.