Abstract: Genetic diversity and genetic relationships of 17 A. macrocephala germplasms from different place were studied by ISSR markers.The results showed that 9 polymorphic primers were selected, and the average percentage of polymorphic bands was 72%.The genetic similarity coefficient（GS） and genetic distance（GD） of various germplasms ranged from 0.589 to 0.904 and 0.084 to 0.425,respectively.Further, the 17 germplasms were divided into 7 groups by cluster analysis.Among them, B 3（namely Pingjiang-2 from Hunan Povince） and B 17（namely Zheshu 1） had the closest genetic relationship, while B 5（from Xiaocun village, Xianfeng County, Hubei Province） and B 14（from Huishan village, Xinchang County, Zhejiang Province） had the farthest genetic relationship.In conclusion, the 17 A. macrocephala germplasms had high genetic diversity and rich genetic information.