Abstract: In order to excavate the types of maize germplasm resources and their utilization potential, genetic diversity analysis, principal component analysis and cluster analysis were carried out on the agronomic traits of 36 maize lines from the Huang-Huai-Hai summer maize regional experiment in 2017.The results showed that the genetic diversity index of yield trait was the highest with an average of 1.800,and the genetic diversity index of resistance to pests and diseases was the lowest with an average of 1.453 4,among which the genetic diversity index of the southern rustwas only 0.346.The contribution rate of the first four principal components（yield, growth period, plant height and spike height） to variation was 58.64%.Cluster analysis divided the tested lines into 3 categories and 6 subcategories.41.2% of the tested lines were distributed in the same group as the control group, which indicated that the tested lines had less germplasm similarity with the dominant varieties popularized in production.