Abstract: In this study, the single factor experiment was carried out to study the survival rate and growth status of Dioscorea alata.treated with 3 kinds of hypertonic substances, and the results showed that at 150 days of culture, the average height of plants treated with 10 g·L^-1 sucrose was（3.55±0.17） cm, which was significantly lower than that of the control and other treatments. In addition, the average height of plants treated with 30 g·L^-1 and 40 g·L^-1 mannitol was（3.88±0.19） cm and（2.13±0.19） cm, respectively, which were significantly lower than that of the control.Comprehensively considering the height, survival rate and growth status of plants, the treatment with 30 g·L^-1 was significantly better than that treated with 40 g·L^-1. Also, in the range of 0-40 g·L^-1,there was no significant difference in plant height and survival rate compared with the control with the increase of sorbitol concentration. Both 10 g·L^-1 sucrose and 30 g·L^-1 mannitol treatment could effectively inhibit the growth of tissue culture seedlings of D. alata within 150 days.