Abstract: In order to understand the fatty acid composition of corn with micro-endosperm for oil, 2 micro-endosperm maize inbred lines with high oleic acid content and 6 micro-endosperm maize inbred lines with medium low oleic acid content were randomly crossed to create 12 micro-endosperm maize hybridized combinations based on NCⅡ design. The fatty acid composition of the virgin oil was determined and its combining ability and genetic parameters was also studied. The results showed that the contents of oleic acid, linoleic acid, linolenic acid, palmitic acid and stearic acid were 42.67%—47.80%, 31.90%—40.93%, 0.64%—0.79%, 10.85%—14.50%, 3.08%—4.44%, respectively, and the contents of seven fatty acids were less than 1%, including linolenic acid, arachidonic acid, arachidonic acid, behenic acid, tetracosanoic acid, palmitoenoic acid and heptadecanoic acid. The variance of general combining ability（GCA） and specific combining ability（SCA） of oleic acid, linoleic acid, stearic acid and lignotaric acid were significant, and the relative effect value of total combining ability（TCA） was in the same order as the measured value of each character. And, the variance of GCA of linolenic acid, palmitic acid, stearic acid and palmitic monoenoic acid was larger, and the additive effect played the main role. The variance of the SCA of linoleic acid content and tetracosanoic acid content was larger, and the non-additive effect played the main role. Finally, the variance of GCA of oleic acid content was 52.56%, which was influenced by both additive effect and non-additive effect.