Abstract: Absract:In order to investigate distribution characteristics of vernalization and photoperiod-related genes and their relationship with yield in new wheat varieties in Henan province, four vernalization-related loci（Vrn-A1, Vrn-B1, Vrn-D1 and Vrn-B3） and one photoperiod-related loci（Phd-D1） were detected by STS markers in 118 wheat varieties from the regional test area of winter wheat varieties in Henan province in recent years. Combined with the agronomic traits of the tested materials, the composition of vernalization-related genes of new wheat varieties in Henan province in recent years and its correlation with winter-spring characteristic, seedling-heading period and yield of the varieties were also analyzed. The results showed that the loci of Phd-D1、Vrn-A1、Vrn-B3 were recessive allelic variation. There were 9 varieties contained dominant allelic variation of Vrn-B1 loci, with a distribution frequency of 7.62%, and all of them showed weak vernalization. Additionally, there were 41 varieties contained dominant allelic variation of Vrn-D1 with a distribution frequency of 34.7%, and 21 of these varieties showed weak vernalization. Further analysis showed that the dominant allelic variation of Vrn-Dl loci was negatively correlated with seedling-heading stage and positively correlated with yield components, but not significantly. And, the dominant allelic variation of Vrn-Bl loci was negatively correlated with productive tiller number and kernels per spike, and positively correlated with 1000-grain weight, but but not significantly.