Abstract: In this paper, an endophyte with broad-spectrum resistance to disease was isolated from the root of wild Bletilla striata in Guizhou province. It was designated GZFJ 015 and belonged to a fungus of the genus Chaetomium sp. In order to investigate the inhibitory activity of the tested strains against the pathogens from fungus rods of Lentinula edodes Ganoderma lucidum under the conditions of imitated ecological culture, the effects of fermentation liquid from strain GZFJ 015 on inhibitory rate and indoor virulence of mycelial growth of pathogens of different edible fungi was determined based on growth rate method. And, the effects of temperature and pH on the antibacterial stability of fermentation broth was further studied. The results showed that the fermentation broth from GZFJ 015 had inhibitory effects on the mycelial growth of L. edodes pathogens XG 1, XG 2, XG 3 and G. lucidum pathogens LZ 1, LZ 2 and LZ 3, and the inhibitory effect on LZ 2 was the most significant（p＜0.05）, up to 85.1% while the inhibition rates of other pathogens ranged from 66.4% to 83.8%. The effective medium concentration of fermentation broth(EC₅₀) was 11.84, 77.91, 6.28, 29.65, 13.72, 48.23 μL·mL^-1, respectively. The toxicity to pathogens XG 3, LZ 1, XG 2 and LZ 3 was better than that of carbendazim. Moreover, the antimicrobial activity of the fermentation broth was relatively stable under 80 ℃ and the pH was in the range of 6.0-11.0.