Abstract: The Capsicum annuum L. is not only an important vegetable and seasonings, but also one of the leading industries in Guizhou. However, cadmium（Cd） pollution in cultivated land severely restricts the development of the pepper industry in recent years. In this paper, the effects of CdCl₂ stress on seed germination and physicochemical indexes of pepper varieties LCL 4 and LCL 6, which were selected for cadmium tolerance, were studied. The results showed that under 200 mg·L^-1 CdCl₂ stress, the germination rate of LCL 4 and LCl 6 seeds was 97% and 96%, while the germination potential was 95% and 93.33%, respectively, indicating that CdCl₂ stress had a great effect on the germination index of LCL 6 seeds. And, the activities of antioxidantenzymes（AOES） were significantly different between the two pepper cultivars treated with 150 mg·L^-1 CdCl₂ stress. The activities of AOES in LCL 4 and LCL 6 increased at first and then decreased, and the activity of SOD, POD and CAT reached the maximum at 3 day of Cd stress. The maximum values of CAT, POD and SOD in LCL 6 were 235.21, 981.32 and 756.25 U·（g·min）^-1, respectively, while those in LCL 4 were 252.16, 1116.05 and 913.73 U·（g·min）^-1, respectively. In addition, MDA content of LCL 6 was significantly higher than that of LCL 4 during 0—3 days of cadmium stress, but the MDA content of LCL 4 and LCL 6 was 6 μmol·g^-1 and 5 μmol·g^-1, respectively, with no significant difference. The above research provided theoretical basis for further screening Cd-tolerant pepper varieties and exploring the mechanism of Cd tolerance.