Abstract: In this paper, the germination characteristics of Bletilla striata under six Ca²⁺ treatment concentrations(0,1.65,2.47,3.3,4.12,4.95 g·L^-1) were studied.The effects of different Ca²⁺ concentrations on seed germination and procorm development were also investigated by plant tissue culture.The results showed that the seeds of B. striata first broke the seed coat under the treatment with 2.47 g·L^-1 Ca²⁺ solution after 10 days of aseptic seeding.Additionally, seed leaves grew better under 3.3 g·L^-1 Ca²⁺ solution after 20 d aseptic seeding, while seed young leaves grew better under 2.47 g·L^-1 and 3.3 g·L^-1 Ca²⁺ solution.After 15 d of aseptic sowing, the seed germination rate was calculated, and the highest germination rate was found when the treatment with concentration of Ca²⁺ was 2.47 g·L^-1,followed by 0 and 1.65 g·L^-1,when the concentration of Ca²⁺ was more than 2.47 g·L^-1,the seed germination was inhibited.After 30 d of aseptic seeding, the diameter of B. striata procorm was larger under 4.95 g·L^-1 Ca²⁺ solution.