Abstract: The problem of infection of Lentinus edodes rod during the production of L. edodes in Guizhou province is very serious. In order to find out the pathogen of L. edodes and its infection process to L. edodes mycelium, a similar strain（No.GZCC 21-0004） was obtained from five production bases of L. edodes in Guizhou province as the experimental material and studied.The results showed that the pathogen was identified as Trichoderma lentinulae of the complex species of Trichoderma harzianum based on morphological characteristics and fractionation bionomics.The process of Trichoderma infestation on the mycelia of L. edodes was studied by plate confrontation, slide confrontation and metabolite inhibition, and the results showed that infecting process of L. edodes with T. lentinulae could be divided into five steps, namely growth of attachment, wrapping, separation of chromatin, rupture of apex, and shrinkage of middle space.And, the results showed that the inhibitory rates of T. lentinulae mycelia, metabolites and volatiles to L. edodes mycelia were 51%,57% and 82%,respectively, and the inhibitory rate of volatiles was the highest.