Abstract: In this paper, the 27 rose varieties introduced and preserved were selected as experimental materials to analyze the mainly varietal characteristics of the varieties combined with the characteristics of rose and actual production. Synchronously, 15 indexes of roses in three aspects, such as ornamental ability, adaptability and resistance, and economic traits were also investigated by consulting experts, and Analytic Hierarchy Process（AHP） was used to give weight to its traits, and 27 rose varieties were analyzed and screened. Finally, a comprehensive evaluation system of rose germplasm resources was established based on the above research, and suitable rose varieties were further screened out. The results showed that most of the 27 introduced rose varieties could be adapted to cultivation in Guizhou. The rose variety Fenghua appeared excellent performance in all aspects, and 11 varieties including Kushui, Liangye and Zizhi had also excellent performance. And, the established rose evaluation system coulf comprehensively evaluate the introduced rose germplasms. Therefore, the evaluation system of rose germplasm resources played an important guiding role in the collection of rose germplasm resources, breeding of new varieties and promotion of cultivation.