Abstract: In order to establish the technical system of quality evaluation and establish the quality grading standards of Ligusticum Chuanxiong Lingzhong, the authenticity, purity, clarity, morphological indexes and viability of 30 stems and 60 species of L. Chuanxiong Lingzhong were tested by referring to the Chinese Crop Seed Inspection Regulations. Meanwhile, the main indexes and reference indexes were analyzed by mathematical statistics method, and the classification standard was established by K-means clustering method. The results showed that the first grade of the Lingzhong belonged to Zhengshan series, with the diameter of the node plate≥20 mm, the Lingzi ratio≥2.5 and the clarity≥98%, while the second grade of the Lingzhong also belonged to Zhengshan series, with the diameter of the node plate between 15 to 20 mm, the Lingzi ratio between 2.0 to 2.5 and the clarity≥95%. And, the Lingzi belonged to Tulingzi, with the diameter of the node plate≥13 mm, the Lingzi ratio≥2.0 and the clarity≥95%. Additionally, the third grade of the Lingzhong belonged to Zhengshan series, with the diameter of the node plate between 13 to 15 mm, the Lingzi ratio≥1.8 and the clarity≥95%, while the rest of the Lingzhong belonged to unqualified ones. To sum up, the quality grading standard of L. Chuanxiong Lingzhong was preliminarily established.