Abstract: To clarify the genetic basis of stripe rust resistance of spring wheat variety Ningchun 27 in Ningxia at adult-plant stage, the hybrid inbred lines of Ningchun 4 and Ningchun 27 were used as materials to analyze the genetic characteristics of stripe rust resistance in Ningchun 27 based on genetic model method of major genes for quantitative traits in plants combined with polygenetic mixing.The results showed that stripe rust resistance of Ningchun 27 belonged to the resistant type of adult-plant stage, and the resistant type in RILs population was distributed continuously, being a quantitative traits. According to the genetic analysis of responding type and severity of the disease, Ningchun 27 contained two pairs of main genes for resistance to stripe rust at adult-plant stage, and the main genes showed different modes of action. The optimal genetic model of responding type was 2 MGDuplicate while the optimal model of severity was 2 MG-Inhibiting. Moreover, the Ningchun 27 contained three pairs of main genes for resistance to stripe rust at adult-plant stage based on the analysis of prevalence rate, and the optimal genetic model was 3 Mg-AI.