Abstract: By investigating the agronomic traits, growth period and resistance of 97 barley germplasms, the germplasms with excellent comprehensive traits was selected to provide basis for high-yield breeding of barley.And, multivariate statistical methods such as correlation coefficient analysis, cluster analysis and principal component analysis were used to analyze the genetic diversity of six traits.The results showed that the average coefficient of variation of 6 traits was 14.45%,the variable coefficient of grain number and subpanicle node length was the largest, while the variable coefficient of protein content was the lowest.Further, the 97 materials were divided into 4 groups, namely group Ⅰ belonging to group with short rod, group Ⅱ belonging to group low protein content, group Ⅲ belonging to group with high rod and high protein content, and group Ⅳ including 48 materials with excellent yield and traits by systematic clustering.In principal component analysis, the first four principal components with 89.9% cumulative variance contribution rate could be selected to evaluate 97 barley germplasms.In this study, phenotypic specificity and genetic diversity of different barley resources were revealed, and some excellent resources were screened out, which provided important scientific basis for breeding new barley varieties with high yield and good quality.