Abstract: In this study, twelve maize inbred lines were used to construct 36 combinations by incomplete diallel cross, and the combining ability of 11 main traits such as yield per plant was also analyzed. The results showed that the general combining ability（GCA） of P 18-7 and Dan 340 was higher in the male parents, while the GCA of P 18-12 and P 18-13 was higher in the female parents.In breeding, maize inbred lines with higher effect value of GCA can produce superior hybrid offspring.The crosses with higher special combining ability were P 18-13 × P 18-7, P 18-15 × Dan 340,P 18-10 × W 527,P 18-13 × P 1811,P 18-14 × P 1811 and P 18-14 × Su 37.Among them, the male inbred line P 18-7 with outstanding GCA was crossed with the female inbred line P 18-13 with high GCA,and the P 18-13 × P 18-7 combination had relatively high special combining ability.It was preliminarily judged that this combination had application value and was worth further testing, screening and utilization.