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高山植物威氏绿绒蒿种子表型及萌发特性研究

Study on Seed Phenotype and Germination Characteristics of lpine Plant Meconopsis wilsonii

  • 摘要: 以珍稀濒危植物威氏绿绒蒿(Meconopsis wilsonii)种子为研究对象,利用扫描电镜和体视镜观察种子微形态特征,同时测定种子的含水量、吸水率以及生理生化指标。采用外源激素浸泡种子的方法,研究不同浓度GA3、6-BA、SA和NAA处理对威氏绿绒蒿种子萌发的影响。结果表明,威氏绿绒蒿种子近肾形,种皮纹饰为褶皱型;种子处于正常状态且具有活力,具备萌发潜力;在所设激素浓度范围内,使用800 mg·L-1 GA3处理时效果最好,种子发芽率和发芽势均为最高,分别为93.33%和27.56%;NAA浓度为80 mg·L-1时,种子发芽率和发芽势均为最高,分别为82.22%和18.89%;6-BA浓度为1 mg·L-1时发芽率最高,为73.33%,在浓度为20 mg·L-1和40 mg·L-1时显著降低了发芽率和发芽势(p<0.05);SA浓度为25 mg·L-1时发芽率和发芽势均为最高,分别为74.44%和13.33%,在浓度为800 mg·L-1时显著降低了发芽率和发芽势(p<0.05)。

     

    Abstract: In this paper, the seeds of Meconopsis wilsonii, a rare and endangered plant, were studied by scanning electron microscopy(SEM) and stereoscopy to observe the morphological characteristics of the seeds, and determine the water content, water absorption and physiological and biochemical indexes of the seeds. And, the effects of different concentrations of GA3, 6-BA, SA and NAA on seed germination of M. wilsonii were studied by soaking seeds with exogenous hormones. The results showed that the seeds of M. wilsonii were nearly kidney shape, and the ornamentation of seed coats was pleated. The seeds were in normal state and had vigor and germination potential. In the range of hormone concentration, the treatment with 800 mg·L-1 GA3 had the best effect, and the germination rate and germination potential of seeds were the highest, being 93.33% and 27.56% respectively. In addition, seed germination rate and germination potential were the highest which were 82.22% and 18.89% respectively while NAA concentration was 80 mg·L-1. The highest germination rate was 73.33% while 6-BA concentration was 1 mg·L-1, and the germination rate and germination potential were significantly decreased while 6-BA concentration was 20 mg·L-1 and 40 mg·L-1(p<0.05). The germination rate and germination potential were the highest which were 74.44% and 13.33% respectively while SA concentration was 25 mg·L-1, and the germination rate and germination potential were significantly decreased(p<0.05) while SA concentration was 800 mg·L-1.

     

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