Abstract: In this study, twenty Panicum miliaceum materials were selected as the research subjects to investigate the effects of drought stress on seed germination, seedling growth and physiological indexes of P. miliaceum by simulate drought stress with 20% PEG-6000 solution. The results showed that there were differences in drought resistance among the 20 P. miliaceum at germination stage. Under drought stress, the seedling growth of 20 P. miliaceum was inhibited to varying degrees, and the plant height and root length were lower than those of the control（except TL and ML）. Seedling growing faster underground than above ground, while RWC showed a decreasing trend compared with the control, and the decreasing rate was different among different materials. The content of MDA and Pro in leaves was higher than that in the control, and the increase was different among different materials, but the content of MDA in XY and TG leaves was lower than that in the control.