Abstract: In order to protect and utilize the wild resources of Dendrobium flexicaule, the seeds of naturally pollinated D. flexicaule were used as research materials, and the sterile germination characteristics of seeds of D. flexicaule were studied by tissue culture, and the effects of combination of basic medium and exogenous additives on seed germination were also explored, so as to screen out the optimal substrate for germination culture. The results showed that the seed germination of D. flexicaule underwent four stages, namely imbibition stage, procorm-formation stage, leaf-formation stage and rooting stage. The germination rate of D. flexicaule with 1/2 N₆ as the basic medium and mashed potato as the exogenous additive reached 98.8%, and the germination rate was the fastest, and the germination could start within 14 days, and the seedlings formed after 84 days. The results of this study provide a reference for the conservation and rapid propagation of D. flexicaule in vitro.