农杆菌介导几种不同马铃薯外植体转化研究

Study on Agrobacterium tumefaciens-mediated Transformation of Several Potato Explants

摘要: 以黄金薯微型薯和中薯8号微型薯,中薯8号无菌苗茎段和Desiree无菌苗茎段为外植体,利用根癌农杆菌介导FAD2基因转化马铃薯,并对影响转化因素进行优化。结果表明,转化植株经PCR检测分析,最终获得黄金薯微型薯阳性植株3株,转化效率为13.3%;中薯8号微型薯、中薯8号无菌苗茎段、Desiree无菌苗茎段各2株,转化效率分别为25%、15.4%、25%。确定FAD2基因已整合到马铃薯基因组中。用马铃薯茎段转化比用马铃薯微型薯转化效率高,且工作量和污染相对较少。

Abstract: In this paper, the FAD2 gene was transformed into potato by Agrobacterium tumefaciens-mediated with the stem segments of the tissue culture plantlet of Huangjinshu, minituber of Zhongshu 8, Zhongshu 8 and Desiree as explants. The results showed that 3 positive minituber plants of Huangjinshu were obtained by PCR detection and analysis, and the conversion efficiency was 13.3%. There were 2 positive plants from the minituber of Zhongshu 8, stem segments of Zhongshu 8 and Desiree respectively, with transformation efficiency of 25%, 15.4%, 25%. The results showed that the FAD2 gene was confirmed to be integrated into the potato genome. Compared with the microtuber, the transformation efficiency of potato stem segment was higher, and the work was less and the pollution was less.