Abstract: In this study, the 33 imported sweet potato germplasm resources were selected as experimental materials to study the genetic relationship among the materials based on morphological marker clustering analysis, and further for providing reference for the breeding of new sweet potato varieties in Guizhou. The results showed that 33 materials could be divided into two groups in the Euclidean cluster 9.71. Group I included 31 materials and was divided into two subgroups, while Group I included 2 materials. The difference of traits in the same group was small and Euclidean distance was similar, while there were great differences in traits and Euclidean distance among populations.